{"id":6280,"date":"2016-09-29T09:40:12","date_gmt":"2016-09-29T08:40:12","guid":{"rendered":"http:\/\/www.fundacionisabelgemio.com\/fr\/?page_id=6280"},"modified":"2017-12-28T19:34:33","modified_gmt":"2017-12-28T18:34:33","slug":"caracterisation-de-cellules-avec-un-potentiel-myogenique","status":"publish","type":"page","link":"https:\/\/www.fundacionisabelgemio.com\/fr\/que-faisons-nous\/caracterisation-de-cellules-avec-un-potentiel-myogenique","title":{"rendered":"Caract\u00e9risation de cellules avec un potentiel myog\u00e9nique"},"content":{"rendered":"\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_section-1410f7e6be3791a849a7730ec4d02faf\">\n.avia-section.av-av_section-1410f7e6be3791a849a7730ec4d02faf{\nbackground-repeat:no-repeat;\nbackground-image:url(https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/02\/IMG_0096_15.jpg);\nbackground-position:0% 0%;\nbackground-attachment:scroll;\n}\n.avia-section.av-av_section-1410f7e6be3791a849a7730ec4d02faf .av-section-color-overlay{\nopacity:0.7;\nbackground-color:#2e2e2e;\n}\n<\/style>\n<div id='av_section_1'  class='avia-section av-av_section-1410f7e6be3791a849a7730ec4d02faf main_color avia-section-default avia-no-shadow  avia-builder-el-0  el_before_av_layout_row  avia-builder-el-first  avia-full-stretch avia-bg-style-scroll av-section-color-overlay-active container_wrap sidebar_right'  data-section-bg-repeat='stretch'><div class=\"av-section-color-overlay-wrap\"><div class=\"av-section-color-overlay\"><\/div><div class='container av-section-cont-open' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-6280'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_heading-0c4ac75f440eabdfb525428bda73f3b6\">\n#top .av-special-heading.av-av_heading-0c4ac75f440eabdfb525428bda73f3b6{\npadding-bottom:0;\ncolor:#ffffff;\n}\nbody .av-special-heading.av-av_heading-0c4ac75f440eabdfb525428bda73f3b6 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-av_heading-0c4ac75f440eabdfb525428bda73f3b6 .special-heading-inner-border{\nborder-color:#ffffff;\n}\n.av-special-heading.av-av_heading-0c4ac75f440eabdfb525428bda73f3b6 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-av_heading-0c4ac75f440eabdfb525428bda73f3b6 av-special-heading-h2 custom-color-heading blockquote modern-quote modern-centered  avia-builder-el-1  avia-builder-el-no-sibling '><h2 class='av-special-heading-tag'  itemprop=\"headline\"  >Caracterizaci\u00f3n de c\u00e9lulas con potencial miog\u00e9nico<\/h2><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<\/div><\/div><\/main><!-- close content main element --><\/div><\/div><\/div><div id='av-layout-grid-1'  class='av-layout-grid-container av-av_layout_row-caf1f26e08707a2ebe9176b630e736c2 entry-content-wrapper main_color av-flex-cells  avia-builder-el-2  el_after_av_section  avia-builder-el-last  grid-row-not-first  container_wrap sidebar_right'  >\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_cell_three_fourth-5227a743201b625eadc00b27db60eab9\">\n.flex_cell.av-av_cell_three_fourth-5227a743201b625eadc00b27db60eab9{\nvertical-align:top;\n}\n.responsive #top #wrap_all .flex_cell.av-av_cell_three_fourth-5227a743201b625eadc00b27db60eab9{\npadding:30px 30px 30px 30px !important;\n}\n<\/style>\n<div class='flex_cell av-av_cell_three_fourth-5227a743201b625eadc00b27db60eab9 av-gridrow-cell av_three_fourth no_margin  avia-builder-el-3  el_before_av_cell_one_fourth  avia-builder-el-first ' ><div class='flex_cell_inner'><section  class='av_textblock_section av-av_textblock-0366cc7376be6c9e82a3e9cc8987b64f'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><p style=\"text-align: center;\">Gracias a muchas personas hemos aportado <strong>418.000 \u20ac<\/strong> a esta l\u00ednea de investigaci\u00f3n<\/p>\n<\/div><\/section><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_hr-a6de236e6c183bbbd700292deacd2b52\">\n#top .hr.hr-invisible.av-av_hr-a6de236e6c183bbbd700292deacd2b52{\nheight:20px;\n}\n<\/style>\n<div  class='hr av-av_hr-a6de236e6c183bbbd700292deacd2b52 hr-invisible  avia-builder-el-5  el_after_av_textblock  el_before_av_textblock '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n<section  class='av_textblock_section av-av_textblock-0366cc7376be6c9e82a3e9cc8987b64f'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><p class=\"p1\"><span class=\"s1\">Nuestro laboratorio est\u00e1 trabajando en cinco l\u00edneas relacionadas con las enfermedades neuromusculares: 1) En primer lugar, tratamos de establecer modelos celulares humanos derivados de pacientes con Distrofia de Duchenne y distrofia de cinturas 2A, que permitan el estudio de la fisiopatolog\u00eda de ambas enfermedades, 2) Estamos trabajando tambi\u00e9n en el estudio espec\u00edfico de la regulaci\u00f3n del calcio dentro de la fibra muscular de estas enfermedades y en la s\u00edntesis y \u00a0desarrollo precl\u00ednico de compuestos que puedan ser f\u00e1rmacos utilizables en todas las enfermedades musculares donde se afecte esta regulaci\u00f3n, 3) Estamos trabajando en la expresi\u00f3n g\u00e9nica en la distrofia muscular tipo 2A, tratando de descubrir nuevas v\u00edas alteradas en relaci\u00f3n con la matriz extracelular y su posible regulaci\u00f3n farmacol\u00f3gica, 4) trabajamos en colaboraci\u00f3n con las \u00e1reas de Onvolog\u00eda y Medicina Regenerativa de nuestro Instituto en la caracterizaci\u00f3n de los procesos de envejecimiento celular asociados a la distrofia miot\u00f3nica o enfermedad de Steinert y por \u00faltimo, 5) Estamos trabajando en el estudio del sustrato metab\u00f3lico de la esclerosis lateral amiotr\u00f3fica, enfermedad que se sit\u00faa en el \u00e1rea de solapamiento entre la neurodegeneracion central y la neuromuscular.<\/span><\/p>\n<p class=\"p1\"><span class=\"s1\">Disponemos de modelos celulares derivados de muestras humanas de piel y m\u00fasculo asi como de diversos animales (mdx, NSG mdx-nude, c3KO) asi como de c\u00e9lulas miog\u00e9nicas obtenidas mediante reprogramaci\u00f3n celular de fibroblastos o mediante sobreexpresi\u00f3n de un factor de transcripci\u00f3n espec\u00edfico de m\u00fasculo, MyoD. En paralelo, hemos creado y optimizado matrices de soporte con biomateriales que mejoren la eficiencia de los cultivos musculares <em>in vitro<\/em>.<\/span><\/p>\n<\/div><\/section><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_hr-5ea7596238ed9f191e6b5e662093052f\">\n#top .hr.hr-invisible.av-av_hr-5ea7596238ed9f191e6b5e662093052f{\nheight:30px;\n}\n<\/style>\n<div  class='hr av-av_hr-5ea7596238ed9f191e6b5e662093052f hr-invisible  avia-builder-el-7  el_after_av_textblock  el_before_av_one_full '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n<div class='flex_column av-av_one_half-2a9015ff38129c418a3f2eafba3e9512 av_one_half first flex_column_div '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_image-79cc5a9c11dc0abaf168b22e4e55a313\">\n.avia-image-container.av-av_image-79cc5a9c11dc0abaf168b22e4e55a313 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-av_image-79cc5a9c11dc0abaf168b22e4e55a313 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-av_image-79cc5a9c11dc0abaf168b22e4e55a313 av-styling- avia-align-center'   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" class='wp-image-16367 avia-img-lazy-loading-not-16367 avia_image ' src=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.04.13.png\" alt='' title='Cultivos de c\u00e9lulas musculares'  height=\"425\" width=\"567\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.04.13.png 567w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.04.13-300x225.png 300w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.04.13-450x337.png 450w\" sizes=\"(max-width: 567px) 100vw, 567px\" \/><\/div><\/div><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8\">\n#top .av_textblock_section.av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8 .avia_textblock{\ncolor:#333333;\n}\n<\/style>\n<section  class='av_textblock_section av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock av_inherit_color'  itemprop=\"text\" ><p><em>Fotograf\u00eda de cultivos de c\u00e9lulas musculares derivados de c\u00e9lulas de la piel. Los fibroblastos que han sobreexpresado MyoD se han convertido en c\u00e9lulas musculares, las cuales se han fusionado para formar miotubos y expresan marcadores musculares, como por ejemplo la cadena pesada de la miosina, la cual se visualiza en verde en la imagen de fluorescencia que se muestra.<\/em><\/p>\n<\/div><\/section><\/p><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_half-9c1d454dc06f7247226bc4e8f931daae\">\n.flex_column.av-av_one_half-9c1d454dc06f7247226bc4e8f931daae{\n-webkit-border-radius:0px 0px 0px 0px;\n-moz-border-radius:0px 0px 0px 0px;\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div class='flex_column av-av_one_half-9c1d454dc06f7247226bc4e8f931daae av_one_half flex_column_div av-zero-column-padding '     ><p>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_image-0da36bde8ed2146aa7aef1386d3a7758\">\n.avia-image-container.av-av_image-0da36bde8ed2146aa7aef1386d3a7758 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-av_image-0da36bde8ed2146aa7aef1386d3a7758 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-av_image-0da36bde8ed2146aa7aef1386d3a7758 av-styling- avia-align-center'   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img decoding=\"async\" class='wp-image-16371 avia-img-lazy-loading-not-16371 avia_image ' src=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.10.16.png\" alt='' title='Fotograf\u00eda de cultivos de c\u00e9lulas musculares'  height=\"433\" width=\"575\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.10.16.png 575w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.10.16-300x226.png 300w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/Captura-de-pantalla-2015-05-26-a-las-14.10.16-450x339.png 450w\" sizes=\"(max-width: 575px) 100vw, 575px\" \/><\/div><\/div><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8\">\n#top .av_textblock_section.av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8 .avia_textblock{\ncolor:#333333;\n}\n<\/style>\n<section  class='av_textblock_section av-av_textblock-34ab98c25efa3b06a3e65f63ca3152b8'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock av_inherit_color'  itemprop=\"text\" ><p><em>Fotograf\u00eda de cultivos de c\u00e9lulas musculares derivados de rat\u00f3n en la matriz que hemos desarrollado. La formaci\u00f3n de fibras, as\u00ed como la madurez que han alcanzado son \u00f3ptimas, lo que se refleja en el gran n\u00famero de miotubos formados y las estriaciones que presentan. La inmunofluorescencia muestra los miotubos, que expresan la cadena pesadade miosina, en verde, y los nucleos, en azul.<\/em><\/p>\n<\/div><\/section><\/p><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_hr-5ea7596238ed9f191e6b5e662093052f\">\n#top .hr.hr-invisible.av-av_hr-5ea7596238ed9f191e6b5e662093052f{\nheight:30px;\n}\n<\/style>\n<div  class='hr av-av_hr-5ea7596238ed9f191e6b5e662093052f hr-invisible'><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_full-9497bbf8bff73ea4c6992cc17fa9ec36\">\n.flex_column.av-av_one_full-9497bbf8bff73ea4c6992cc17fa9ec36{\n-webkit-border-radius:0px 0px 0px 0px;\n-moz-border-radius:0px 0px 0px 0px;\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div class='flex_column av-av_one_full-9497bbf8bff73ea4c6992cc17fa9ec36 av_one_full  avia-builder-el-8  el_after_av_hr  el_before_av_hr  first flex_column_div av-zero-column-padding '     ><div  class='avia-button-wrap av-av_button-cb48beb3374e192316e6a860b7412260-wrap avia-button-left  avia-builder-el-9  avia-builder-el-no-sibling '><a href='http:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2016\/10\/caracterizacion-de-celulas-con-potencial-miogenico.pdf' class='avia-button av-av_button-cb48beb3374e192316e6a860b7412260 avia-icon_select-yes-left-icon avia-size-medium avia-position-left avia-color-theme-color' target=\"_blank\" rel=\"noopener noreferrer\"><span class='avia_button_icon avia_button_icon_left' aria-hidden='true' data-av_icon='\ue84b' data-av_iconfont='entypo-fontello'><\/span><span class='avia_iconbox_title' >Consultar los resultados<\/span><\/a><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_hr-5ea7596238ed9f191e6b5e662093052f\">\n#top .hr.hr-invisible.av-av_hr-5ea7596238ed9f191e6b5e662093052f{\nheight:30px;\n}\n<\/style>\n<div  class='hr av-av_hr-5ea7596238ed9f191e6b5e662093052f hr-invisible  avia-builder-el-10  el_after_av_one_full  el_before_av_one_half '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n<div class='flex_column av-av_one_half-2a9015ff38129c418a3f2eafba3e9512 av_one_half  avia-builder-el-11  el_after_av_hr  el_before_av_one_half  first flex_column_div '     ><div  class='avia-testimonial-wrapper av-av_testimonials-dfdcbe2bbda75220796248d01d289b52 avia-grid-testimonials avia-grid-1-testimonials avia_animate_when_almost_visible' >\n<section class=\"avia-testimonial-row\"><div class='avia-testimonial av-av_testimonial_single-31eda84d4c72b1d4d943dda7957c6fdc avia-testimonial-row-1 avia-first-testimonial flex_column no_margin av_one_full'><div class='avia-testimonial_inner'  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia-testimonial-image'  itemprop=\"image\" ><img loading=\"lazy\" decoding=\"async\" width=\"180\" height=\"180\" src=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde-180x180.jpg\" class=\"attachment-square size-square\" alt=\"Dr. Adolfo L\u00f3pez de Munain\" srcset=\"https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde-180x180.jpg 180w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde-80x80.jpg 80w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde-36x36.jpg 36w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde-120x120.jpg 120w, https:\/\/www.fundacionisabelgemio.com\/wp-content\/uploads\/2015\/05\/adolfolopezde.jpg 250w\" sizes=\"auto, (max-width: 180px) 100vw, 180px\" \/><\/div><div class='avia-testimonial-content '><div class='avia-testimonial-markup-entry-content'  itemprop=\"text\" ><\/div><\/div><div class=\"avia-testimonial-meta\"><div class=\"avia-testimonial-arrow-wrap\"><div class=\"avia-arrow\"><\/div><\/div><div class='avia-testimonial-meta-mini'  itemprop=\"author\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/Person\" ><strong  class='avia-testimonial-name'  itemprop=\"name\" >Dr. Adolfo L\u00f3pez de Munain<\/strong><span  class='avia-testimonial-subtitle '  itemprop=\"jobTitle\" >Investigador Jefe<\/span><span class='hidden avia-testimonial-markup-link'  itemprop=\"url\" >manually,http:\/\/www.fundacionisabelgemio.com\/fr\/propos-la-fondation\/dr-adolfo-lopez-de-munain<\/span><span class=\"avia-testimonial-subtitle-sep\">&ndash;<\/span><a class='aviablank avia-testimonial-link' href='manually,http:\/\/www.fundacionisabelgemio.com\/fr\/propos-la-fondation\/dr-adolfo-lopez-de-munain' rel=\u2019noopener noreferrer\u2019>Mas informacion<\/a><\/div><\/div><\/div><\/div><\/section>\n<\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_one_half-9c1d454dc06f7247226bc4e8f931daae\">\n.flex_column.av-av_one_half-9c1d454dc06f7247226bc4e8f931daae{\n-webkit-border-radius:0px 0px 0px 0px;\n-moz-border-radius:0px 0px 0px 0px;\nborder-radius:0px 0px 0px 0px;\npadding:0px 0px 0px 0px;\n}\n<\/style>\n<div class='flex_column av-av_one_half-9c1d454dc06f7247226bc4e8f931daae av_one_half  avia-builder-el-13  el_after_av_one_half  avia-builder-el-last  flex_column_div av-zero-column-padding '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-av_image-90f03872e369a6b77119061681132030\">\n.avia-image-container.av-av_image-90f03872e369a6b77119061681132030 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-av_image-90f03872e369a6b77119061681132030 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-av_image-90f03872e369a6b77119061681132030 av-styling- avia-align-center  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